MicroRNA-125b: association with disease activity and the treatment response of patients with early rheumatoid arthritis

نویسندگان

  • Veronika Hruskova
  • Romana Jandova
  • Lucia Vernerova
  • Herman Mann
  • Ondrej Pecha
  • Klara Prajzlerova
  • Karel Pavelka
  • Jiri Vencovsky
  • Maria Filkova
  • Ladislav Senolt
چکیده

BACKGROUND MicroRNAs (miRNAs) are small RNAs that regulate gene expression by targeting mRNA. It was proved that some miRNAs are significantly deregulated in rheumatoid arthritis (RA). MicroRNA-125b negatively regulates expression of TNF-α, which plays a crucial role in RA pathogenesis. The aim of this study was to determine the treatment outcome of patients with early RA based on the expression of circulating and cellular miR-125b. METHODS Total RNA was isolated from the plasma and peripheral blood mononuclear cells (PBMCs) of 58 patients with early RA before and three months after treatment initiation and of 54 age- and sex-matched healthy controls (HC). The expression of miR-125b was measured by TaqMan quantitative PCR. The treatment responders were defined as patients achieving remission or low disease activity (28-joint count disease activity score (DAS28) <3.2). Receiver operating characteristic (ROC) curve and stepwise backward multivariable logistic regression analyses of miR-125b expression were used to predict the disease outcome at three and six months after initiation of treatment. RESULTS The expression of miR-125b in the PBMCs and plasma of treatment-naïve early RA patients was significantly lower than that of HC and increased significantly after three months of treatment, particularly in responders. However, only the cellular expression of miR-125b was inversely correlated with disease activity. MiR-125b expression in PBMCs was higher in responders than in non-responders after three months (p = 0.042). Using ROC analysis, the cellular expression of miR-125b, but not the disease activity at baseline, predicted the treatment response after three months of therapy (area under the curve 0.652 (95 % CI 0.510 to 0.793); p = 0.048). CONCLUSION The expression of miR-125b in PBMCs of treatment-naïve patients may present a novel biomarker for monitoring the treatment outcome during the early phase of RA.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

25(OH) vitamin D serum values and rheumatoid arthritis disease activity (DAS28ESR)

Background: The role of vitamin D in the pathogenesis of rheumatoid arthritis is under investigation. This study was designed to evaluate the correlation between serum values of 25(OH) vitamin D [25(OH)D] and disease activity in rheumatoid arthritis (RA) patients according to Disease Activity Score 28 joints and ESR (DAS28ESR). Methods: Ninety-nine patients according to ACR classification crit...

متن کامل

The Association between Depression and vitamin D level with disease activity in patient with rheumatoid arthritis

Introduction: Rheumatoid arthritis (RA) is a chronic rheumatologic disease, affecting different aspects of patient life. Although several studies have reported a higher rate of depression among patients with RA, there is still controversy over the underlying risk factors and probable covariates of depression in these individuals. The present study investigated the relationship between disease a...

متن کامل

Periodontitis in rheumatoid arthritis patients, abundance and association with disease activity

Background: There are some discrepancy in association between activity of rheumatoid arthritis (RA) and periodontitis. The aim of this study was to evaluate the periodontal status of outpatients with RA. &nbsp;&nbsp; Methods: The study was conducted in 2013-14 in a rheumatology clinic in Sari, north of Iran on 74 patients with RA.&nbsp; Evaluation of RA disease activity was according to diseas...

متن کامل

The roles of sex, Perceived Pain and illness Perceptions on Disease Activity in Rheumatoid Arthritis

Background: In the “treating to target” strategy to rheumatoid arthritis (RA) management, “cognitive” beyond “physical” measures allow a more comprehensive assessment. This study reports a predictive analysis of patient properties on Disease Activity, and the degree to which these predictions could be uniquely attributable to Illness perception (IP), pain, and sex differences. Methods: This cr...

متن کامل

Association between obesity types and anthropometric indices with rheumatoid arthritis disease

Background: Obesity is one of the causes of onset and exacerbation of rheumatoid arthritis. However, limited studies have examined the association between body composition and severity of disease. The aim of this study was to evaluate the association between severity of rheumatoid arthritis with various types of obesity and the comparison of anthropometric indices between patients with rheumato...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 18  شماره 

صفحات  -

تاریخ انتشار 2016